This will copy each sequence out of the list into a separate document, while retaining the original sequence within the list. To extract sequences from a list, select the sequence(s) you want to extract and go to Sequence → Extract Sequences from List. Note that this copies your sequences into a list and retains the original sequence documents. To existing sequences in your database into a list, or combine two lists into one, select the sequences or sequence lists you want to group and go to Sequence → Group Sequences into a List. When you import files containing multiple sequences you will be asked if you want to store those sequences in a list. Sequence lists make it easier to manage large numbers of sequences by grouping related sequences into a single document. This will create a new sequence document containing the selected sequence. To create a new sequence from an existing sequence, select the region of sequence that you want then click the Extract button above the sequence viewer, or go to Sequence → Extract Regions. Bases not in the (green) binding region will be included as a 5′ extension. If your primer contains a 5′ extension, you can specify this by setting the length of the binding region. If your sequences are oligonucleotides, choose Primer or Probe as the type. You can change this by clicking the Type option. Geneious will automatically determine whether your sequence is nucleotide or protein based on the composition of the bases you enter. Here you can paste or type in the residues for your new sequence, then enter the Name, Description and Organism for your sequence if required. New sequences can be imported from existing files as described in Importing and Exporting Data or they can be created manually by going to Sequence → New Sequence, or File → New → Sequence. This study is the first molecular characterization of mbpa from the native species Piaractus brachypomus.Creating, Viewing and Editing Sequences Creating new sequences Conclusions The physicochemical computed features agree with the biological functions of MBP, and basal gene expression was according to the anatomical distribution in the tissues analyzed. Gene expression of mbpa was upregulated in the optic chiasm of the chlorpyrifos exposed fish in contrast to the control group. MBPa was recognized as belonging to the myelin basic protein family, closely related with orthologous proteins, and two intrinsically disordered regions were established within the sequence. Results mbpa complete open reading frame was identified with 414 nucleotides distributed in 7 exons that encode 137 amino acids. Gene expression was assessed by qPCR in three models corresponding to sublethal chlorpyrifos exposure, acute brain injury, and anesthesia experiments.
Bioinformatic tools were used to identify the phylogenetic relationships, physicochemical characteristics, exons, intrinsically disordered regions, and conserved domains of the protein. Methods The aim of this study was to molecularly characterize the myelin basic protein a (mbpa) gene from the Colombian native fish, red-bellied pacu, Piaractus brachypomus. Likewise, MBP has been proposed as a marker of demyelination in traumatic brain injury and chemical exposure. MBP has several functions including organization of the myelin membranes, reorganization of the cytoskeleton during the myelination process, and interaction with the SH3 domain in signaling pathways. Abstract : Background Myelin basic protein (MBP) is one of the most important structural components of the myelin sheaths in both central and peripheral nervous systems.
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